Some security tools inspect files with static signatures to determine if they are known malicious. Adversaries may add data to files to increase the size beyond what security tools are capable of handling or to change the file hash to avoid hash-based blacklists.
Identify potentially malicious software that may be executed from a padded or otherwise obfuscated binary, and audit and/or block it by using whitelisting  tools, like AppLocker,   or Software Restriction Policies  where appropriate. 
Depending on the method used to pad files, a file-based signature may be capable of detecting padding using a scanning or on-access based tool.
When executed, the resulting process from padded files may also exhibit other behavior characteristics of being used to conduct an intrusion such as system and network information Discovery or Lateral Movement, which could be used as event indicators that point to the source file.
- Foltýn, T. (2018, March 13). OceanLotus ships new backdoor using old tricks. Retrieved May 22, 2018.
- Counter Threat Unit Research Team. (2017, October 12). BRONZE BUTLER Targets Japanese Enterprises. Retrieved January 4, 2018.
- Grunzweig, J. (2018, January 31). Comnie Continues to Target Organizations in East Asia. Retrieved June 7, 2018.
- FireEye. (2015). APT28: A WINDOW INTO RUSSIA’S CYBER ESPIONAGE OPERATIONS?. Retrieved August 19, 2015.
- Falcone, R. and Miller-Osborn, J.. (2016, February 3). Emissary Trojan Changelog: Did Operation Lotus Blossom Cause It to Evolve?. Retrieved February 15, 2016.
- FinFisher. (n.d.). Retrieved December 20, 2017.
- Allievi, A.,Flori, E. (2018, March 01). FinFisher exposed: A researcher’s tale of defeating traps, tricks, and complex virtual machines. Retrieved July 9, 2018.
- Symantec Security Response Attack Investigation Team. (2018, April 23). New Orangeworm attack group targets the healthcare sector in the U.S., Europe, and Asia. Retrieved May 8, 2018.
- Axel F, Pierre T. (2017, October 16). Leviathan: Espionage actor spearphishes maritime and defense targets. Retrieved February 15, 2018.
- Haq, T., Moran, N., Scott, M., & Vashisht, S. O. (2014, September 10). The Path to Mass-Producing Cyber Attacks [Blog]. Retrieved November 12, 2014.
- Lunghi, D., et al. (2017, December). Untangling the Patchwork Cyberespionage Group. Retrieved July 10, 2018.
- ESET. (2016, October). En Route with Sednit - Part 2: Observing the Comings and Goings. Retrieved November 21, 2016.
- Schwarz, D., Sopko J. (2018, March 08). Donot Team Leverages New Modular Malware Framework in South Asia. Retrieved June 11, 2018.
- Huss, D., et al. (2017, February 2). Oops, they did it again: APT Targets Russia and Belarus with ZeroT and PlugX. Retrieved April 5, 2018.
- Beechey, J. (2010, December). Application Whitelisting: Panacea or Propaganda?. Retrieved November 18, 2014.
- Tomonaga, S. (2016, January 26). Windows Commands Abused by Attackers. Retrieved February 2, 2016.
- NSA Information Assurance Directorate. (2014, August). Application Whitelisting Using Microsoft AppLocker. Retrieved March 31, 2016.
- Corio, C., & Sayana, D. P. (2008, June). Application Lockdown with Software Restriction Policies. Retrieved November 18, 2014.
- Microsoft. (2012, June 27). Using Software Restriction Policies and AppLocker Policies. Retrieved April 7, 2016.